Journal: bioRxiv

Article Title: Cold Tolerance is Governed by Diverse Genetic Mechanisms Including Those Regulated by NB-LRR-type Receptor Proteins in Arabidopsis

doi: 10.1101/2022.01.18.476799

Figure Lengend Snippet: Schematic representation of the steps taken in identifying genes involved in cold stress using a high-throughput phenotyping platform. After suspending in low melting agarose (a) , seeds were stratified, and sown (b), and grown in the Arabidopsis growth chambers fitted with digital cameras (c) . Two-dimensional images of the rosette leaves of individual genotypes were captured and analyzed using Matlab GUI software (Method S2) (d) . The digital images were converted to pixel data for conducting easyGWAS to identify putative cold-stress-related genes. The candidate cold-stress-related genes were validated by studying knockout mutants. Homozygous knockout mutants were identified (e) . SALK_104944, knockout T-DNA insertion mutant for AT1G68320 ; HM, homozygous line; HZ, heterozygous line; WT (Col-0 ecotype), wild-type with no insertion. Knockout mutants were phenotyped to determine if any of the 33 putative cold-stress-related genes play a role in cold tolerance (f) .

Article Snippet: Two-dimensional images of the rosette leaves of individual mutants were captured and analyzed by Matlab GUI software (Method S2).

Techniques: High Throughput Screening Assay, Software, Knock-Out, Mutagenesis